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Anti-SRGAP1 Rabbit pAb (100 μl)
| Reactivity: | H & H |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SRGAP1 rabbit polyclonal |
Gene Name: | SLIT-ROBO Rho GTPase-activating protein 1 |
Synonyms: | ARHGAP13, KIAA1304, SRGAP1, Rho GTPase-activating protein 13 |
Immunogen: | Recombinant protein corresponding to Mouse SRGAP1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 121 kDa |
Observed MW. | 121 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | HepG2, Jurkat, 293T |
IHC | Human | 1: 300-1: 600 | esophageal cancer, colon, placenta |
Background
SRGAP1 (Slit-robo Rho GTPase activating protein 1), also known as ARHGAP13 (Rho GTPase activating protein 13), functions as a GTPase-activating protein for Cdc42 and Rho A. Expressed in kidney, testis, lung and brain, SRGAP1 contains an FCH (Fes/CIP4 homology) domain, a Rho-GAP domain and an SH3 domain. In the presence of Slit, SRGAP1 (via its SH3 domain) binds to the CC3 motif in robo (a protein responsible for mediating the repulsive effect of Slit) with higher affinity and inhibits Cdc42 activity in a robo/SRGAP-dependent manner.
Images
| Western blot analysis of SRGAP1 (GB113414) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SRGAP1 (GB113414) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded human colon using SRGAP1 (GB113414) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded human placenta using SRGAP1 (GB113414) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
