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Anti-SUPT3H Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SUPT3H rabbit polyclonal antibody |
Gene Name: | Transcription initiation protein SPT3 homolog |
Synonyms: | SPT3-like protein, SUPT3H, SPT3 |
Immunogen: | Recombinant protein corresponding to Mouse SUPT3H |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 36 kDa |
Observed MW. | 36 kDa |
Uniprot ID: | O75486 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | pancreas |
IHC/IF | Mouse, Rat | 1: 500-1: 1500 | brain, heart |
Background
The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from delta sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription.
Images
| Western blot analysis of SUPT3H (GB112125) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded mouse brain using SUPT3H (GB112125) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse heart using SUPT3H (GB112125) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using SUPT3H (GB112125) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded rat heart using SUPT3H (GB112125) at dilution of 1: 1500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
