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Anti-Smac / Diablo Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Diablo homolog, mitochondrial |
Synonyms: | Diablo, DIABLO, DFNA64, SMAC, Diablo, Diablo homolog, diablo IAP-binding mitochondrial protein, Smac |
Immunogen: | Recombinant protein corresponding to Mouse Smac |
Uniprot ID: | Q9JIQ3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 27 kDa / 21 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, brain, kidney |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa, NIH-3T3 |
Background
Diablo homolog (DIABLO) is a mitochondrial protein encoded by the DIABLO gene. SMAC is a mitochondrial protein that promotes cytochrome c- and TNF receptor-dependent activation of apoptosis by inhibiting the effect of IAP–a protein that negatively regulates apoptosis, or programmed cell death. SMAC is normally a mitochondrial protein localized to the mitochondrial intermembrane space, but it enters the cytosol when cells undergo apoptosis.
Images
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Western blot analysis of Smac (GB11896) at dilution of 1: 500 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using Smac(GB11896) at dilution of 1: 100-1: 400 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using Smac(GB11896) at dilution of 1: 100-1: 400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
