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Anti-Smad2 + Smad3 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Mothers against decapentaplegic homolog 2 |
Synonyms: | MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD2, MADH2, MADR2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Smad2 + Smad3 |
Uniprot ID: | Q62432 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 49/52 kDa / 49-60 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, HepG2, NIH/3T3 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1500 | lung, ovary, stomach, ovarian cancer |
Background
SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255.
Images
| Western blot analysis of Smad2/3 (GB111844) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human ovarian cancer using Smad2/3 (GB111844) at dilution of 1: 400 |
 | Immunohistochemistry analysis of paraffin embedded mouse lung injury using Smad2/3 (GB111844) at dilution of 1: 400 |
 | Immunohistochemistry analysis of paraffin embedded rat lung injury using Smad2/3 (GB111844) at dilution of 1: 400 |
 | Immunohistochemistry analysis of paraffin embedded mouse ovary using Smad2/3 (GB111844) at dilution of 1: 400 |
 | Immunohistochemistry analysis of paraffin embedded rat ovary using Smad2/3 (GB111844) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin embedded human stomach using Smad2/3 (GB111844) at dilution of 1: 400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
