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Anti-Ube2N / Ubc13 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Ubiquitin-conjugating enzyme E2 N |
Synonyms: | UbCH ben, Ube2N, ubiquitin carrier protein N, ubiquitin protein ligase N, BLU, Bendless-like ubiquitin-conjugating enzyme, MGC131857, MGC8489 |
Immunogen: | Recombinant protein corresponding to Mouse Ube2N |
Uniprot ID: | P61089 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 17 kDa / 17 kDa |
Images
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis, small intestine, spinal cord |
IHC/IF | Human, Mouse, Rat | 1: 250-1: 1000 | colon cancer, colon, small intestine |
Background
UBC13 is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. Existing as a heterodimer with UBE2V2, UBC13 catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. Additionally, UBC13 mediates the transcription of several target genes and is thought to play a role in cell cycle progression, cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage.
Images
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Western blot analysis of ubc13 (GB11986) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded mouse spinal cord using ubc13 (GB11986) at dilution of 1: 600 |
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Immunohistochemistry analysis of paraffin embedded human colon using ubc13 (GB11986) at dilution of 1: 600 |
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Immunohistochemistry analysis of paraffin embedded human colon cancer using ubc13 (GB11986) at dilution of 1: 600 |
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Immunofluorescent analysis of paraffin embedded rat spinal cord using ubc13 (GB11986) at dilution of 1: 500 |
| Immunofluorescent analysis of paraffin embedded rat small intestine using ubc13 (GB11986) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
