LiFluor™ Cell Viability Kits are principally used to enumerate the proportion of live and dead cells in a population. The fluorescence-based cell viability and cytotoxicity assays are less hazardous and less expensive than radioisotopic techniques, more sensitive than colorimetric methods and more convenient than animal testing methods. Our cell viability and cytotoxicity assays are reliable and easy to perform for simultaneous quantitation of live and dead cells. Our kits are suitable for detection with a fluorescence microscope, flow cytometer or microplate reader, and ideal for high-throughput screening applications.
Key Features
• Simplicity: Reagents are simultaneously added, no wash steps are required.
• Specificity and reliability: Distinct color for live and dead cell.
• Versatility: Suitable for fluorescence microscope, flow cytometer or microplate reader.
LiFluor™ Cell Viability Imaging Kit is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of Hoechst 33342 Live Cell Stain reagent and Green 488 Dead Cell Stain reagent to 1 ml of cell growth media, then determine viability by counting total vs dead cells. Hoechst 33342 reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. Green 488 Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC (green) filter set. This kit is appropriate for fluorescence microscopy and flow cytometry.
Product Name | Cat. # | Price | View |
LiFluor™ Green Cell Viability Imaging Kit (2×10 ml) | C0030 | $149 | |
LiFluor™ Red Cell Viability Imaging Kit (2×10 ml) | C0031 | $149 |
Case Study
Detection of live or dead cells from animal cells (left), bacteria cells (middle), and yeast (right) using the LiFluor™ Cell Viability Kits.