LiFluor™ Yeast Cell Viability Kit combines a novel two-color fluorescent probe for fungi/yeast viability, Yeast stain, with a fluorescent fungal surface labeling reagent of a third color, Calcofluor White M2R. Calcofluor is an ultraviolet–excitable dye that has long been used as a marker of fungal cell walls. The Yeast stain is used to determine the metabolic activity of fungal cells by fluorescence microscopy or through other instrumental techniques. The assay kit can be used for detecting fungi in complex mixtures or in pure cultures.
Key Features
• Simplicity: Reagents are simultaneously added, no wash steps are required.
• Specificity and reliability: Distinct color for live and dead cell.
• Versatility: Suitable for fluorescence microscope, flow cytometer or microplate reader.
Specifications
1. Platform: Fluorescence Microscopy, Flow Cytometry
2. Detection Method: Fluorescent
3. Ex/Em: 470/590 nm
Applications
Cell viability assay
Components
1. Yeast stain: 300 µl
2. Calcofluor White M2R: 500 µl
Storage
Store at -20°C and protect from light.
Case Study
Live and dead yeast stained with LiFluor™ Yeast Cell Viability Kit.