A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.
Source
PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10mM EDTA.
Specifications
1. Range: 100-1,500 bp
2. Recommended Load: 5 μl / well
3. Containing orange G & xylene cyanol FF as tracking dyes.
Component
1. 100 bp DNA Ladder: 500 µl
Storage
Store at -20°C.
LifeSct LLC is a cutting-edge biotechnology company dedicated to serving life scientists worldwide since 2016. LifeSct's expertise encompasses a comprehensive range of fields such as molecular biology, synthetic biology, cell biology, immunology, proteomics and microbiology.