5-FAM-dUTP is supplied as a 1 mM solution in TE buffer. 5-FAM-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.
Key Features
• Bright signals with low background.
• Wide variety of fluorophores.
• Great for many applications, like Fluorescence in situ Hybridization, TUNEL and more.
Applications
Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.
Specifications
1. Ex/Em: 495/518 nm
2. Molecular Formula: C33H27N3Na3O20P3
3. Molecular Weight: 947.47
Component
1. 5-FAM-dUTP: 25 μl, 1 mM in TE buffer
Storage
Store at -20°C