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Apoptotic, Necrotic & Healthy Cells Detection Kit provides a convenient assay to detect apoptotic, necrotic, and total cells within the same cell population by flow cytometry or fluorescence microscopy. This kit contains three-color reagent: LiFluor™ 488 annexin V used to stain apoptotic cells by binding to phosphatidylserine (PS) exposed on the cell surface; propidium iodide (PI) used to stain necrotic cells with comprised membrane integrity; and Hoechst 33324 used to stain all cell nuclei. After staining a cell population, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and all cells show blue fluorescence.
Key Features
• Simple: Simultaneous detection of apoptotic, necrotic, and total cells in a single assay.
• Fast: A single 30 min staining step.
• Sensitive: Brighter fluorescence signal.
Specifications
1. Platform: Fluorescence Cytometer, Fluorescence Microscopy
2. Detection Method: Fluorescent
3. Ex/Em: PI: 535/617 nm; LiFluor™ 488: 495/520 nm; Hoechst 33324: 350/460 nm
Applications
Cell apoptosis analysis
Components
1. LiFluor 488 Annexin V: 250 µl
2. PI: 250 µl
3. 5Hoechst 33342: 250 µl2. PI: 200 µl
4. 5× Annexin-binding buffer: 25 ml
Storage
Store at 4°C and protect from light.
