Your shopping cart is empty!
BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure total protein concentration compared to a protein standard. The BCA Protein Assay Kit is based on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+ by cysteine, cystine, tryptophan, tyrosine, and peptide bonds. BCA forms a purple colored complex with Cu1+ that is detectable at 562nm, and proportional to the protein present. The BCA Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
Key Features
• Colorimetric: measure with a standard spectrophotometer or plate reader at 562nm.
• Excellent uniformity: less protein-to-protein variation.
• Compatible: unaffected by typical concentrations of most ionic and nonionic detergents.
• Moderately fast: much easier and faster than the Lowry method.
• High linearity: linear working range for BSA equals 20 to 2000 µg/mL
• Sensitive: detect down to 5 µg/mL with the enhanced protocol
Applications
Protein quantitation in solution
Components
1. BCA Reagent A: 500 ml
2. BCA Reagent B: 25 ml
3. BSA standard: 10 ml, 2 mg/ml
Storage
BCA Reagent A and B at RT; BSA Standard at 4°C.
