BCECF, AM is an ideal cell-permeant, dual-excitation ratiometric pH indicator for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.
Specifications
1. Ex/Em: 490/535 nm
2. CAS Number: 117464-70-7
Component
1. BCECF, AM: 1 mg
Storage
Store at -20°C and protect from light.