Carboxy-H2DCFDA (25 mg)

Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent Carboxy-H2DCFDA is converted to the highly fluorescent 5-(and 6)-carboxy-2',7'-dichlorofluorescein, with additional negative charges that impede its leakage out of the cell.
 

Specifications

1. Ex/Em: 495/529 nm

2. Detection Method: Fluorescent

3. Molecular Formula: C25H16Cl2O9

4. Molecular Weight: 531.30
 

Applications

Probe for ROS
 

Component

1. Carboxy-H2DCFDA: 25 mg
 

Storage

Store at -20°C and protect from light.