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CCK-8 Cell Counting Kit allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. The highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. The amount of the formazan dye, generated by the activities of dehydrogenases in cells, is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Key Features
• Quick and Simple Protocol
• High sensitivity
Specifications
1. Platform: Microplate Reader
2. Detection Method: Colorimetric
3. OD: 450 nm
Applications
1. Cell proliferation analysis
2. Cell viability assays, including cytotoxicity
3. Cytokine assays
Component
1. CCK-8 Solution: 5 ml
Storage
Store at 4°C and protect from light.
Case Study
