Your shopping cart is empty!
The LiFluor™ 488 EdU Flow Cytometry Assay Kit offers a streamlined and robust approach to analyzing DNA replication in proliferating cells, surpassing the traditional BrdU cell proliferation assay in both simplicity and reliability. In this advanced assay, EdU, a modified thymidine analogue, is efficiently incorporated into the DNA of newly synthesized cells and then labeled with a bright, photostable LiFluor™ 488 dye through a swift and highly-specific click reaction. The fluorescent tagging of these proliferating cells is not only precise but also compatible with antibody methods, thanks to the gentle nature of the click protocol. The analysis of newly synthesized DNA is facilitated using the 488 nm laser line of the flow cytometer, ensuring accurate and efficient detection.
Key Features
• Simple: Labeling is complete in two steps.
• Accurate: Superior results compared to BrdU assay.
• Fast: Results in as little as 90 minutes.
• Multiplex: Compatible with cell cycle dyes and antibody-based stain.
Specifications
1. Platform: Fluorescence Microscope
2. Detection Method: Fluorescent
3. Ex/Em: 495/520 nm
Applications
Cell proliferation analysis by flow cytometer
Components
1. EdU: 2×1 ml
2. LiFluor 488 azide: 150 µl
3. LiFlour fixative: 5 ml
4. Permeabilization and wash reagent: 50 ml
5. CuSO4: 1 ml
6. EdU buffer additive: 200 mg
Storage
Store at -20°C and protect from light.
Case Study
Cell proliferation analysis with LiFluor™ 488 EdU Flow Cytometry Assay Kit. Jurkat cells were treated with 10 µM EdU for 2 hours and detected according to the recommended staining protocol. The figures show a clear separation of proliferating cells which have incorporated EdU and nonproliferating cells which have not.
