Your shopping cart is empty!
The LiFluor™ 488 EdU Flow Cytometry Assay Kit provides a highly efficient and reliable method for analyzing DNA replication in proliferating cells. It outperforms the traditional BrdU cell proliferation assay in terms of both simplicity and reliability. In this state-of-the-art assay, EdU, a modified thymidine analogue, is seamlessly incorporated into the DNA of newly dividing cells. Subsequently, a vibrant and photostable LiFluor™ 488 dye is attached to the EdU through a rapid and highly specific click reaction. Due to the mild nature of the click protocol, the fluorescent labeling of these proliferating cells is not only extremely accurate but also fully compatible with antibody-based techniques. The analysis of newly synthesized DNA is effortlessly carried out using the 488 nm laser line of the flow cytometer, guaranteeing precise and efficient detection.
Key Features
• 90-Minute Protocol: 70% faster than BrdU immunodetection
• Dual-Laser Compatibility: Optimized for 488nm & 405nm excitation systems.
• Multi-Parameter Analysis - Simultaneous cell cycle staining (PI/DAPI) + surface markers.
Specifications
1. Platform: Fluorescence Microscope
2. Detection Method: Fluorescent
3. Ex/Em: 495/520 nm
Applications
Cell proliferation analysis by flow cytometer
Components
1. EdU: 2×1 ml
2. LiFluor 488 azide: 150 µl
3. LiFlour fixative: 5 ml
4. Permeabilization and wash reagent: 50 ml
5. CuSO4: 1 ml
6. EdU buffer additive: 200 mg
Storage
Store at -20°C and protect from light.
Case Study
Cell proliferation analysis with LiFluor™ 488 EdU Flow Cytometry Assay Kit. Jurkat cells were treated with 10 µM EdU for 2 hours and detected according to the recommended staining protocol. The figures show a clear separation of proliferating cells which have incorporated EdU and nonproliferating cells which have not.
