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| Key Features: | • Simple: Labeling is complete in two steps • Efficient: No denaturation steps or harsh treatment required • Content-rich results: Better preservation of cell morphology, antigen structure, and DNA integrity • Consistent: Not dependent on variable antibody lots for detection |
| Description: | LiFluor™ 488 EdU Imaging Kit is a superior alternative to the traditional BrdU cell proliferation assay that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable LiFluor™ dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol. |
| Specifications: | 1. Platform: Fluorescence Microscope 2. Detection Method: Fluorescent 3. Ex/Em: 495/520 nm |
| Applications: | Cell proliferation analysis in vitro and in vivo. |
| Component: | 1. Component A: EdU (2×1 ml) 2. Component B: LiFluor 488 azide (100 µl) 3. Component C: EdU reaction buffer (50 ml) 4. Component D: CuSO4 (1 ml) 5. Component E: EdU buffer additive (200 mg) 6. Component F: Hoechst 33342 (70 µl) |
| Storage: | At -20°C and Protect from light |
| Download: | Manual |
Detection of cell proliferation in cell. A549 cells were treated with 10 μM EdU for 2 hr, then detected with LiFluor™ 488 azide (green), cells were counterstained with DAPI (blue).
Detection of cell proliferation in mouse tissue. A mouse was injected intraperitoneally with 5 mg EdU per kilogram body weight, then sacrificed at 0, 12, and 24 hr. The mouse intestine tissue was detected with LiFluor™ 488 azide (green), and counterstained with DAPI (blue).
