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The EndoFree Midi Plasmid Kit adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA.The special Buffer EBT and the Filtration CS1 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 1 hour, which is convenient and quick.
Key Features
• Suitable for extracting plasmids from 50-100 ml bacteria culture medium.
• The purified plasmid contain very low endotoxin and can meet the requirements of high quality transfection.
• Easy to operate with a unique and rapid endotoxin removal method.
Specifications
| Plasmid Type | Bacterial Culture Volume | Yield | Plasmids |
| Low Copy | 50 ml | 20-50 μg | pBR322, pACYC, pSC101, SuperCos, pWE15 |
| High Copy | 20 ml | 80-250 μg | pTZ, pUC, pBS, pGM-T |
Components
1. RNase A (10 mg/ml): 150 μl
2. Buffer BL: 30 ml
3. Buffer P1: 30 ml
4. Buffer P2: 30 ml
5. Buffer E3: 15 ml
6. Buffer EBT: 70 ml
7. Buffer GDE: 30 ml
8. Buffer MRDE: 36 ml
9. Buffer PWF: 16 ml
10. Buffer TB: 15 ml
11. Filtration CS1: 10
12. Spin Columns CP7: 10
13. Collection Tubes 15 ml: 20
Storage
Store at room temperature.
