SuperLumin™ Firefly Luciferase Assay Kit provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideally suited for high-throughput applications, the simple workflow provides robust performance and also eliminates many of the handling inconveniences experienced using other reporter assays. The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media.
Key Features
• No centrifugation or pre-lysis steps required. Reagents are added directly to growth medium.
• Stable signal allows flexibility for batch or continuous processing of 96- and 384-well plates.
• Simple protocol is compatible with any luminometer.
Components
1. Dual Firefly Luciferase Substrate: 100 μl, 100×
2. Dual Firefly Luciferase Buffer: 10 ml
Storage
Store at 4°C.