JC-1 Dye (1 mg)

JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 exists as a monomer at low concentrations and yields green fluorescence, similar to fluorescein. At higher concentrations, the dye forms J-aggregates that exhibit a broad excitation spectrum and an emission maximum at ~590 nm. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green.
 

Specifications

1. Ex/Em: 514/529 nm; 585/590 nm

2. Molecular Formula: C25H27Cl4IN4

3. Molecular Weight: 652.23

4. CAS Number: 47729-63-5
 

Component

1. JC-1 dye: 1 mg
 

Storage

Store at 2-8°C and protect from light.