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The Lentiviral Packaging Kit is meticulously engineered to streamline the packaging of third-generation lentivectors into viral particles capable of expressing your lentiviral construct across a broad spectrum of mammalian cells. This advanced 3rd generation packaging system is designed with the highest standards of biosafety in mind. The lentiviral Rev gene is provided as a separate vector, distinct from other structural genes, which effectively eliminates the risk of reverse recombination and the generation of replication-competent viral particles. This feature ensures that your lentiviral applications are both potent and secure.
Key Features
• Effectively leverage the powerful lentiviral transduction platform with optimized HIV-based packaging system.
• Easily generate high-titer lentiviral preparations.
• Efficiently deliver most third-generation lentivector expression constructs.
Components
1. Packaging Plasmid Mix: 50 μl (0.5 μg/μl)
2. Control Plasmid: 50 μl (0.25 μg/μl)
3. PrimeFect™ Transfection Reagent: 250 µl
Storage
Packaging plasmid mix and control plasmid: at -20°C; Transfection reagent: at 4°C
