Lentivirus Transduction Enhancer II (1 ml)

Lentivirus Transduction Enhancer II is a nontoxic novel chemical reagent that increases lentiviral transduction efficiency by modulating membrane permeability. Normally, incubation of lentivirus particles in cells in the presence of polybrene is generally efficient in transducing many cell types. However, due to polycation property, polybrene is toxic for some sensitive cells. Lentivirus Transduction Enhancer II is a neutral and nontoxic transduction enhancer, which significantly increases lentivirus transducing efficiency while maintaining cell membrane integrity.

Lentivirus Transduction Enhancer II is most effective when added to cell culture media at the time of transduction. Recommended working concentration ranges from 1:100 to 1:50. However, the working concentration is highly cell line-dependent. Lower or higher dilution ratio may be required to optimize the effect.