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LiPure™ Exosome DNA Purification Kit is designed for the isolation of exosomal DNA from pure exosome isolated by our Exosome Isolation Kits. The kit also features a buffer system that facilitates complete exosome lysis for efficient nucleic acid isolation. Exosomal DNA is bound to spin column, washed and eluted. Normally, 100-300 ng of exosomal DNA can be purified from the exosome isolated from 200 μl blood serum or 5 ml cell media. The extracted DNA is ready for downstream applications such as sequencing, and PCR. No need to precipitate, concentrate or desalt.
Key Features
• Rapid: The purification process takes only 30 minutes.
• Fully compatible: The isolated DNA can be used in most downstream applications such as PCR and sequencing.
• Good stability: The optimized buffer system provides consistent results.
• Clean: Minimal carryover of co-precipitating proteins.
Applications
Exosomal DNA isolation from serum, plasma, urine, cell culture media, and other body fluids.
Technical Specifications
1. Sample Input: 5 ml cell culture media, 200-500 μl blood serum
2. Elution Volume: 30-100 μl
3. Technology: Silica spin column
Sample Source
Serum, plasma, urine, cell culture media, and other body fluids.
Components
1. Buffer AL: 8 ml
2. Buffer DW1: 6 ml
3. Buffer DW2: 10 ml
4. Buffer AE: 10 ml
5. Mini Column: 25
6. 2 ml Collection Tube: 25
Storage
Store at room temperature.
