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LiPure™ cfDNA Purification Kit provides a rapid and easy method for the isolation of circulating DNA from plasma, serum, and other cellular body fluids. The kit allows for simultaneous processing of single or multiple samples in under 2 hours. The cfDNA Purification Kit uses a specially formulated buffer system to lyse sample under denaturing conditions, then the lysis was transferred to the DNA column where DNA binds to column matrix and cellular debris, hemoglobin, and other proteins are washed away. The isolated circulating DNA is ready for applications such as PCR, microarrays, and next generation sequencing.
Key Features
• Rapid: DNA purification process in less than 2 hr.
• Safe: No Phenol/chloroform extractions.
• High-quality: DNA is suitable for a variety of downstream applications.
Specifications
1. Sample Input: 1-4 ml
2. Elution Volume: 20-50 μl
3. Technology: Silica spin column
Applications
Circulating DNA isolation from plasma, serum, and other cellular body fluids.
Components
1. Buffer ACL: 50 ml
2. Buffer ACB: 50 ml
3. Buffer ACW1: 15 ml
4. Buffer ACW2: 10 ml
5. Buffer AE: 15 ml
6. Proteinase K: 2×1.3 ml, 20 mg/ml
7. Carrier RNA: 250 µl, 0.2 mg/ml
8. Mini Column: 50
9. 2 ml Collection Tube: 50
Storage
Buffer & tube at RT; Proteinase K & Carrier RNA at -20°C
Case Study
Electropherogram of purified cfDNA from serum sample using LiPureTM cfDNA Purification Kit from serum/plasma. Purified cfDNA was analyzed on Agilent’s 2100.
