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The LiQuant™ RNA BR Assay Kit is meticulously designed for the effortless and precise quantification of RNA. This kit excels in its high selectivity for RNA even in the presence of DNA, offering significant advantages in terms of stability, linear dynamic range, and sensitivity over other traditional RNA quantitation methods.
The kit includes a concentrated quantitation reagent, dilution buffer, and pre-diluted RNA standards, simplifying the process of RNA measurement. To use, simply dilute the reagent with the provided buffer, add your sample—accepting any volume from 1 µl to 20 µl—and measure the fluorescence with a fluorescence plate reader or fluorometer. The assay demonstrates excellent tolerance to common contaminants, including proteins, salts, solvents, detergents, and free nucleotides, ensuring reliable results even in complex sample matrices.
Furthermore, the assay's versatility allows it to be adapted for use in microplates, tubes, or cuvettes, making it a flexible tool for various experimental setups and high-throughput applications in RNA quantitation.
Key Features
• Higher sensitivity: 100 times more sensitive than NanoDrop.
• Broad dynamic range: over four orders of magnitude.
• More accuracy: unaffected by the presence of proteins, free nucleotides.
• Easy to use: mix-and-read format.
• Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit.
Applications
RNA quantitation of samples for microarray, RT-PCR, and Northern blot procedures.
Specifications
1. Sample Type: RNA
2. Platform: Fluorescence plate reader or fluorometer
3. Assay: RNA Quantitation, broad range
4. Linear Range: 20-1000 ng RNA
Components
1. LiQuant™ RNA BR Reagent: 200 µl
2. LiQuant™ RNA BR Buffer: 50 ml
3. RNA Standard #1: 200 µl
4. RNA Standard #2: 200 µl
Storage
Store at 2-8°C and protect from light.
Case Study
Left: Quantitation of ribosomal RNA with LiQuant™ RNA Assay Kit using fluorescence plate reader. Right: Quantitation of ribosomal RNA with LiQuant™ RNA Assay Kit using Qubit® Fluorometer.
