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LiQuant™ dsDNA BR Assay Kit provides a simple, sensitive, and accurate quantitation for dsDNA. The kit contains concentrated assay reagent, dilution buffer, and pre-diluted dsDNA standards. The assay kit is highly selective for dsDNA, and highly reliable in detecting dsDNA ranging from 2 to 1000 ng, and offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The assay is performed at room temperature. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 50 µl is acceptable), and read the fluorescenceusing fluorescence plate reader or Fluorometer such as Qubit® or Quantus™ Fluorometer. The kit is well tolerated to common contaminants such as proteins, salts, solvents and detergents.
Key Features
• Higher sensitivity: 100 times more sensitive than NanoDrop.
• Broad dynamic range: over four orders of magnitude.
• More accuracy: unaffected by the presence of proteins, free nucleotides.
• Easy to use: mix-and-read format.
• Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit.
Application
dsDNA quantitation of genomic and miniprep DNA samples
Specifications
1. Sample Type: dsDNA
2. Platform: Fluorescence plate reader or fluorometer
3. Assay: dsDNA Quantitation, broad range
4. Linear Range: 2-1000 ng dsDNA
Components
1. LiQuant™ dsDNA BR Reagent: 200 µl
2. LiQuant™ dsDNA BR Buffer: 50 ml
3. dsDNA Standard #1: 200 µl
4. dsDNA Standard #2: 200 µl
Storage
Store at 2-8°C and protect from light.
Case Study
Left: Quantitation of dsDNA with LiQuant™ dsDNA Assay Kit using fluorescence plate reader. Right: Quantitation of dsDNA with LiQuant™ dsDNA Assay Kit using Qubit Fluorometer.
