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LiScript™ One Step Probe RT-qPCR Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit can prevent the contamination of PCR product. Combining the superior performance of LiScript™ Reverse Transcriptase and hot-start LiTaq™ DNA Polymerase, with an optimized buffering system, the detection sensitivity of LiScript™ One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability. LiScript™ One Step Probe RT-qPCR Kit is provided in Master Mix. The 5× One Step Mix contains an optimized buffer and dNTP Mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).
Key Features
• Higher detection sensitivity.
• Balanced amplification at high and low template concentrations.
• Applicable to multiplex qPCR.
• Better storage stability.
• Compatible with fast mode.
Applications
• The product is suitable for detection of various RNA nucleic acids of animals, plants and microorganisms (viruses, etc.).
Components
1. RNase free ddH2O: 2×1 ml
2. 5× One Step Mix: 600 μl
3. One Step Enzyme Mix: 150 μll
4. 50× ROX Reference Dye 1: 100 μll
5. 50× ROX Reference Dye 2: 100 μl
Storage
Store at -20℃.
