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Lipid peroxidation is the oxidative degradation of cellular lipids by free radicals from reactive oxygen species, which can degrade lipids containing carbon-carbon double bonds such as phospholipids and polyunsaturated fatty acids. Oxidation of lipids leads to the generation of lipid peroxides and can cause damage to cell membranes, resulting in changes to signal transduction pathways and eventually leading to cell death. Lipid peroxidation is involved in apoptosis and one of the main contributors to ferroptosis, an iron-dependent, non-apoptotic form of cell death. Oxidative stress from lipid peroxidation plays a role in aging, as well as pathologies such as cancer, atherosclerosis, and neurodegenerative diseases.
The Lipid Peroxidation Kit enables the detection of lipid peroxidation in live cells through oxidation of BODIPY™ 581/591 C11 reagent. This reagent localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from ∼590 nm to ∼510 nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry. This kit also includes cumene hydroperoxide as a positive control compound to induce lipid peroxidation in cells.
Key Features
• Optimized kit for ratiometric detection of lipid peroxidation in live cells.
• Upon oxidation, the reagent shifts fluorescence emission peak from ~590 nm (red) to ~510 nm (green).
• Cumene hydroperoxide is included as a positive control to induce lipid peroxidation.
• The reagent is provided as a ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy.
Components
1. Lipid Peroxidation Sensor
2. Cumene hydroperoxide
Storage
Store at -20°C and protect from light.
