RPMI-1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI-1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas. RPMI-1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
Specifications
1. Volume: 500 ml
2. Supplementation: Need additional
3. Glutamine: L-Glutamine
4. Phenol Red
5. Antibiotics: No
6. Sodium Pyruvate
7. Sodium Bicarbonate
8. HEPES Buffer: No
9. pH: 7.0-7.6
10. pH: 265~305 mOsmol/kg
11. Endotoxin: <0.3 EU/mL
Component
1. RPMI-1640 Medium: 4×500 ml
Storage
Stored at 2°C~8°C; Avoid repeated freezing and thawing.