TIANgel Purification Kit uses a silicon matrix material that can efficiently and specifically bind DNA and a unique, and this kit can recover DNA fragments from TAE or TBE agarose gel while removing impurities such as proteins, other organic compounds, inorganic salt ions and oligonucleotide primer, etc. The purified DNA fragment range is 100 bp-15 kb with up to 80% of recovery efficiency. Each spin-column can adsorb 10 μg of DNA.DNA recovered with this kit can be used for various operations,including enzyme digestion, PCR, sequencing, library screening, ligation, transformation, etc.
Key Features
• Fast: The entire operation process is fast and convenient, and can be completed in around 10 min..
• Diversity: This kit is capable of recovering single-strand and double-strandDNA segments, as well as ring-shaped plasmid DNA.
• Convenient: Gel blocks can be dissolved at room temperature without the use of balance buffer.
• High efficient: The unique spin column and buffers ensure maximal recovery of high-purity target DNA.
Components
1. Buffer PE: 200 ml
2. Buffer PW: 20 ml
3. Buffer TB: 30 ml
4. Spin Columns CA5 : 200
5. Collection Tubes 2 ml: 200
6. Gel Cutter: 20
Storage
Store at room temperature.
Case Study
Different length of DNA fragments were purified by TIANgel Purification Kit and relevant products from Supplier T, Q and A. 3 μl of the 50 μl eluent was loaded per lane.
Publications
1. The m 6 A modification mediated-lncRNA POU6F2-AS1 reprograms fatty acid metabolism and facilitates the growth of colorectal cancer via upregulation of FASN.
Publication: Molecular Cancer Product: TIANgel Purification Kit
2. Potential for the development of Taraxacum mongolicum aqueous extract as a phytogenic feed additive for poultry.
Publication: Frontiers in Immunology Product: TIANgel Purification Kit
3. Mycoplasma synoviae LP78 is a fibronectin/plasminogen binding protein, putative adhesion, and potential diagnostic antigen.
Publication: Frontiers in Microbiology Product: TIANgel Purification Kit