TIANprep Mini Plasmid Kit (200 preps)

The TIANprep Mini Plasmid Kit adopts the advanced silica membrane adsorption technology to ensure easy and fast operation. The cells are treated by alkaline lysis, and then the plasmid DNA is released and bound to silica membrane. After washing away the impurities, up to 30 μg of plasmid DNA can be efficiently extracted.

This kit provides a unique buffer formulation to remove protein impurities and other organic compounds from a maximum of 1-5 ml of bacteria medium per treatment. Higher purity of DNA can be obtained compared to traditional kits, and the DNA can be directly applied in molecular biology experiments such as enzyme digestion, transformation, sequencing and PCR.
 

Key Features

• Fast: Fewer steps, simpler operation and less time.

• High efficiency: More than 85% of plasmid DNA can be extracted from bacteria.
 

Specifications

Components

1. RNase A (10 mg/ml): 600 μl
2. Buffer BL: 120 ml
3. Buffer P1: 60 ml
4. Buffer P2: 60 ml
5. Buffer P3: 80 ml
6. Buffer PD: 120 ml
7. Buffer PW: 50 ml
8. Buffer EB: 30 ml
9. Spin Columns CP3: 200
10. Collection Tubes 2 ml: 200

Storage

Store at room temperature.

Publications

1. Dynamics of extrachromosomal circular DNA in rice. 
Publication: Nature Communications        Product: TIANprep Mini Plasmid Kit

2. Long-read sequencing of 945 Han individuals identifies novel structural variants associated with phenotypic diversity and disease susceptibility. 
Publication: medRxiv        Product: TIANprep Mini Plasmid Kit

3. Salicylic acid inducing the expression of maize anti-insect gene SPI : a potential control strategy for Ostrinia furnacalis. 
Publication: BMC Plant Biology        Product: TIANprep Mini Plasmid Kit