VAHTS Universal Plus DNA Library Prep Kit for Illumina V2 (24 rxns)

VAHTS Universal Plus DNA Library Prep Kit for Illumina V2 is a DNA library preparation kit featuring enzyme-based fragmentation specifically developed for Illumina high-throughput sequencing platforms. This kit is an upgraded version of the original one. It significantly reduces the proportion of Artificial Invert Chimera Reads in the DNA library of FFPE samples and improves the reliability of the detection of SNV and other biomarkers while maintaining the original high performance. The kit combines DNA fragmentation, end repair, and dA-tailing into one step. The adapter ligation, library amplification, and size selection can be performed directly without additional purification. The 100 pg to 1 μg template DNA can be converted into the library suitable for Illumina high-throughput sequencing platforms. The kit is perfectly compatible with DNA from different sources and ofdifferent input amounts, and the user only needs to adjust the fragmentation time based on the target insert size to obtain a library with the desired fragment size.
 

Key Features

• Lower False-positive Rate: Enhanced accuracy of variant calling with greatly reduced false-positive SNV and InDel calls.

• Excellent Performance: Wide template and input compatibility, good stability, high library conversion rate and output.
 

Applications

1. Whole genome sequencing

2. Whole exome or targeted sequencing

3. Metagenome sequencing

4. Methylation Sequencing
 

Components

1. FEA Buffer V2: 120 µl
2. FEA Enzyme Mix V2: 240 µl
3. Rapid Ligation Buffer V2: 600 μl
4. Rapid DNA Ligase V2: 120 µl
5. VAHTS HiFi Amplification Mix: 600 µl
6. PCR Primer Mix 3 for llumina: 120 µl
7. Neutralization Buffer: 120 µl
8. Control DNA(100 ng/pl): 10 µl
 

Storage

Store at -20°C