5-Aminoallyl-UTP (100 μl)

5-Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as a 10 mM solution in TE buffer. 5-Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled UTP.
 

Key Features

• Bright signals with low background.

• Wide variety of fluorophores.

• Great for many applications, like Fluorescence in situ Hybridization, TUNEL and more.
 

Applications

Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension.
 

Specifications

1. Molecular Formula: C12H17N3Na3O15P3

2. Molecular Weight: 605.17
 

Component

1. 5-Aminoallyl-UTP: 100 μl, 10 mM in TE buffer
 

Storage

Store at -20°C