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LiFluor™ 647 is a bright, far-red fluorescent dye with excitation ideally suited to the 633 nm laser line. LiFluor™ 635 is water-soluable, highly fluorescent and pH-insensitive from pH 4 to pH 10. LiFluor™ 647 can be used as a replacement for Alexa Fluor 647, DyLight 649, Cy5.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting LiFluor™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.
Key Features
• LiFluor™ fluorescent dye has superior brightness and good photostability.
• The dye is insensitive to pH change and has good water solubility.
• Multiplexing: across spectrum from UV to near-IR.
Specifications
1. Ex/Em: 650/666 nm
2. Extinction coefficient: 240,000
3. Molecular weight: ~1,300
4. Excitation source: 633 nm
Applications
Fluorescent labeling
Component
1. LiFluor™ 647 Red Fluorescent Dye (NHS ester): 1 µmol
Storage
Store at -20°C and protect from light.
