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| Key Features: | • Simple workflow: Add, incubate, and read • Flexible: Measure by colorimetric or fluorescent • High sensitivity: Detect as low as 100 cells • Safety: Non-toxic to cells • Low cost and reliable results |
| Description: |
AlamarBlue Cell Viability Kit is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies. |
| Specifications: |
1. Platform: Microplate Reader 2. Detection Method: Colorimetric 3. Ex/EmOD: 530-560/590 nmm |
| Applications: | 1. Cell proliferation analysis 2. Cell viability assays, including cytotoxicity 3. Cytokine assays |
| Component: |
1. AlamarBlue reagent: 25 ml |
| Storage: |
At 4°C and protect from light |
| Download: |
Manual |
Quantitation of Jurkat cells using the AlamarBlue Cell Viability Kit. Jurkat cells were diluted to the indicated cell numbers in 100 µL volumes, delivered to the wells of a microplate, and incubated with AlamarBlue Cell Viability Reagent for 4 hr , fluorescence was measured at Ex/Em=545/590 nm.