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Biotin-14-dCTP is a dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50 µg of DNA.
Key Features
• Convenience and safety: probe preparation generates no radioactive waste.
• Stability: probes remain active longer than isotopically labeled probes.
• Versatility: biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding.
Applications
DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling
Specifications
1. Concentration: 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA.
Component
1. Biotin-14-dCTP: 50 nmol
Storage
Store at -20°C