LiTaq™ DNA Polymerase is a thermostable DNA polymerase that exhibits a 5' → 3' polymerase activity and a 5' →3' exonuclease activity, with no 3' → 5 ' exonuclease activity. LiTaq™ DNA Polymerase is purifed from an Escherichia coli (E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this product. The PCR products contain A at the 3'-end and can be directly cloned into T-Vectors.
Key Features
• The PCR products contain A at the 3'-end and can be directly cloned into T-Vectors.
• The extension rate is about 100bp/sec.
• Amplification of genomic DNA fragment up to 4 kb.
• Very low cost and ideal for genotyping.
Applications
• Routine PCR
• Colony PCR
Components
1. 10× Taq Buffer (Mg2+ plus): 4 ml
2. LiTaq™ DNA Polymerase (5 U/µl): 200 µl
Storage
At -20°C