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LiFluor™ 555 EdU Imaging Kit is a superior alternative to the traditional BrdU cell proliferation assay that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable LiFluor™ dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.
Key Features
• Simple: Labeling is complete in two steps.
• Efficient: No denaturation steps or harsh treatment required.
• Content-rich results: Better preservation of cell morphology, antigen structure, and DNA integrity.
• Consistent: Not dependent on variable antibody lots for detection.
Specifications
1. Platform: Fluorescence Microscope
2. Detection Method: Fluorescent
3. Ex/Em: 555/565 nm
Applications
Cell proliferation analysis in vitro and in vivo.
Components
1. EdU: 2×1 ml
2. LiFluor 555 azide: 100 µl
3. CEdU reaction buffer: 50 ml
4. CuSO4: 1 ml
5. EdU buffer additive: 200 mg
6. Hoechst 33342: 70 µl
Storage
Store at -20°C and protect from light.
Case Study
Detection of cell proliferation in cell. A549 cells were treated with 10 µM EdU for 2 hr, then detected with LiFluor™ 555 azide (red), cells were counterstained with DAPI (blue).
