EndoFree Maxi Plasmid Kit V2 (10 preps)

The EndoFree Maxi Plasmid Kit V2 adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA. By combining the special Endotoxin Removal Buffer ER and Filtration CS1, endotoxin, proteins and other impurities can be effectively removed. The whole extraction process takes only 1 hour, ensuring the convenient and quick operation. The plasmid DNA extracted by this kit can be applied to various routine operations, including enzymatic digestion, PCR,sequencing, ligation, transformation as well as the transfection for various cell types.
 

Key Features

• High purity: The unique endotoxin precipitation technology is adopted to specifically remove endotoxin.

• Easy to operate: The adsorption column technology is used to specifically adsorb plasmid DNA, which makes the operation easier.

• High-efficiency transfection: Suitable for the transfection of most cell lines including endotoxin-sensitive cells.

• Wide range of applications: The purified plasmid can be applied in the transfection of animal and plant cells as well as molecular biology experiments.
 

Specifications

Components

1. RNase A (10 mg/ml): 1.25 ml
2. Buffer BL: 30 ml
3. Buffer P1: 125 ml
4. Buffer P2: 125 ml
5. Buffer P4: 125 ml
7. Buffer ED: 220 ml
8. Buffer PW: 50 ml
8. Buffer TB: 30 ml
9. Endotoxin Removal Buffer ER: 32 ml
10. Filtration CS1: 10
11. Spin Columns CP6: 10
12. Collection Tubes 50 ml: 20

Storage

Store at room temperature.

Case Study

The plasmids purified using EndoFree Maxi Plasmid Kit V2 and thesame products from Supplier 1 and Supplier 2 are eluted with thesame volume of elution buffer. 1 μl plasmid was loaded per lane toestimate the concentration of the plasmid. 100 ng of the plasmidwhose concentration was determined by spectrophotometer wasloaded into the same gel to determine whether the concentration value was falsely high.

Publications

1. In vivo self-assembly and delivery of VEGFR2 siRNA-encapsulated small extracellular vesicles for lung metastatic osteosarcoma therapy. 
Publication: Cell death & disease        Product: EndoFree Maxi Plasmid Kit V2

2. Screening and identification of two pheromone receptors based on the coadaptation of pheromones and their receptors in rats. 
Publication: bioRxiv        Product: EndoFree Maxi Plasmid Kit V2

3. In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis. 
Publication: Nature Communications        Product: EndoFree Maxi Plasmid Kit V2