EpiArt DNA Methylation Library Kit for Illumina V3 (24 rxns)

EpiArt DNA Methylation Library Kit for Illumina V3 for Illumina is a methyl-seq DNA library preparation kit that was specifically developed for the Illumina high-throughput sequencing platform. Based on high-efficiency single strand ligation technology, this kit can convert 10 pg - 250 ng of converted DNA into a sequencing library. It is compatible with DNA samples as short as 40 bp. The optimized library preparation process enables the library preparation to be completed within 2 h, and the manual operation duration is less than 30 min. It is suitable for automatic library preparation and compatible with the capture process. All the reagents in the kit have undergone rigorous quality control and function testing to ensure the optimal stability and repeatability of library preparation.
 

Key Features

• Easy to operate: It takes only 2 hours for a single sample to build a library.

• Wide compatibility: Compatible with 10 pg - 250 ng methylated conversion products.

• Good data quality: Excellent sequencing data quality under different sample types and input conditions.
 

Applications

The kit is suitable for the preparation of llumina high-throughput sequencing platform-specific libraries and is compatible with converted DNA templates. lt is compatible with a variety of sample types: genomic DNA, Formalin-Fixed and Paraffin-Embedded DNA (FFPE DNA).Chromatin immuno-precipitation DNA (ChIP DNA) , cell-free DNA (cfDNA, ctDNA), microbial DNA, etc. lt can be compatible with DNA samples as short as 40 bp. lt can be compatible with 10 pg -250 ng of Input DNA.
 

Components

1. Dilution Buffer: 5 ml
2. 3' Ligation Buffer: 240 µl
3. 3' Ligation Enzyme Mix: 120 µl
4. 3' Adapter: 120 µl
5. Extension Primer: 120 µl
6. Extension Enzyme Mix: 840 µl
7. 5' Ligation Mix: 360 µl
8. 5' Adapter: 120 µl
9. VAHTS HiFi Amplification Mix V3: 600 µl
 

Storage

Store at -20°C

Case Study

1. Input volume compatible with 10 pg - 250 ng

Using 293 cell gDNA as a template, EM101 was used for methylation conversion after fragmentation. The results showed that NE103 could efficiently construct libraries at different starting input amounts (10 pg, 100 pg, 1 ng, 10 ng, 100 ng, 250 ng), and compared with similar products from Supplier A and Supplier B, the library yield was higher and the data quality was better.

2. Compatible with different template types

Different template types (human cfDNA, mouse FFPE DNA, Arabidopsis genomic DNA) were used, and after fragmentation, methylation conversion was performed by EM101. The converted products were constructed according to the standard library construction process of NE103 and similar products of Supplier A and B. The results showed that NE103 can efficiently build libraries.

3. Compatible with different conversion kits

Using 293 cell gDNA as a template, fragmentation was followed by bisulfite conversion and enzymatic conversion kits (Z*, N* company conversion kits) for methylation conversion. The converted products were constructed according to the standard library construction process of NE103, A, and B company similar products. The results showed that NE103 can efficiently construct libraries.