LiFluor™ Red Cell Viability Imaging Kit (2×10 ml)

LiFluor™ Cell Viability Imaging Kit (Blue/Red) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of Hoechst 33342 Live Cell Stain reagent and Propidium Iodide Dead Cell Stain reagent to 1 ml of cell growth media, then determine viability by counting total vs dead cells. Hoechst 33342 reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. PI reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard TRITC/RFP (orange) filter set. This kit is appropriate for fluorescence microscopy and flow cytometry.
 

Specifications

1. Compatible Cells: Eukaryotic Cells, Mammalian Cells

2. Detection Method: Fluorescent

3. Ex/Em: Hoechst 33342 reagent 350/461 nm; Propidium Iodide reagent 535/617 nm
 

Applications

Cell viability assay
 

Components

1. Hoechst 33342 Live Cell Stain: 10 ml
2. Propidium Iodide Dead Cell: 10 ml
 

Storage

Store at 4°C and protect from light.