LiQuant™ RNA HS Assay Kit (200 rxns)

The LiQuant™ RNA HS Assay Kit is meticulously crafted for the straightforward and precise quantification of RNA. This kit is highly selective for RNA, even in the presence of DNA, and surpasses traditional RNA quantitation methods in stability, linear dynamic range, and sensitivity.

The kit comprises a concentrated quantitation reagent, dilution buffer, and pre-diluted RNA standards, streamlining the RNA measurement process. To use, simply dilute the reagent with the provided buffer, add your sample—accepting any volume from 1 µl to 20 µl—and measure the fluorescence with a fluorescence plate reader or fluorometer. The assay is designed to be highly tolerant of common contaminants, including proteins, salts, solvents, detergents, and free nucleotides, ensuring accurate results even in the presence of these potential interfering substances.

Moreover, the assay's adaptability allows for use in microplates, tubes, or cuvettes, making it a versatile tool for various experimental configurations and high-throughput applications in RNA quantitation.
 

Key Features

• Higher sensitivity: 100 times more sensitive than NanoDrop.

• Broad dynamic range: over four orders of magnitude.

• More accuracy: unaffected by the presence of proteins, free nucleotides.

• Easy to use: mix-and-read format.

• Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit.
 

Applications

RNA quantitation of samples for microarray, RT-PCR, and Northern blot procedures.
 

Specifications

1. Sample Type: RNA

2. Platform: Fluorescence plate reader or fluorometer

3. Assay: RNA Quantitation, high sensitivity

4. Linear Range: 5-100 ng RNA
 

Components

1. LiQuant™ RNA HS Reagent: 200 µl
2. LiQuant™ RNA HS Buffer: 50 ml
3. RNA Standard #1: 200 µl
4. RNA Standard #2: 200 µl
 

Storage

Store at 2-8°C and protect from light.
 

Case Study

Left: Quantitation of ribosomal RNA with LiQuant™ HS RNA Assay Kit using fluorescence plate reader. Right: Quantitation of ribosomal RNA with LiQuant™ HS RNA Assay Kit using Qubit® Fluorometer.