LiScript™ One Step Green RT-qPCR Kit (250 rxns)

LiScript™ One Step Green RT-qPCR Kit is specially designed for qPCR detection using RNA (e.g., RNA virus) as templates. Reverse transcription and qPCR can be finished in one tube in the presence of Gene Specific Primers (GSP). There is no additional opening/pipetting operations are required, which greatly increasing assay throughput and reducing the risk of contamination. Intergrating the superior performance of LiScript™ Reverse Transcriptase and LiTaq™ plus DNA Polymerase with the optimized buffer, the lowest RNA template for detection of this kit is 0.1 pg. The specificity-enhancing factor existing in the buffer can effectively reduce the formation of primer dimers and improve the specificity of product. The kit is provided as the formation of master mix, which is convenient to use
 

Key Features

• Excellent specificity: Include a special additive to inhibit primer dimer formation for higher accuracy.  

• High detection sensitivity: High amplification efficiency, allowing detection from 1 pg of total RNA template.
 

Applications

• PCR

• qPCR

• PCR Cloning
 

Components

1. RNase free ddH2O: 2× 1.25 mll
2. 2× One Step Green Mix: 2× 1.25 ml
3. One Step Green Enzyme Mix: 250 μl
4. 50× ROX Reference Dye 1: 100 μl
5. 50× ROX Reference Dye 2: 100 μl
 

Storage

Store at -30 ~ -15°C and transport at ≤ 0°C. For 2× One Step Green Mix, store at -30 ~ -15°C and protect from light.