LiQuant™ DNA/RNA Assay Kits allow precise quantitation of DNA or RNA samples across a wide range of concentrations. Our new LiQuant™ NGS dsDNA assay kit is ideal for quantitating DNA samples in Next Gen Sequencing applications. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.
Selection Guide
Product | Sensitivity | Application |
NGS dsDNA Assay Kit | 0.2-100 ng | dsDNA quantitation of DNA samples for Next Generation Sequencing |
dsDNA HS Assay Kit | 0.2-100 ng | dsDNA quantitation of PCR products, viral DNA and samples for subcloning |
dsDNA BR Assay Kit | 2-1000 ng | dsDNA quantitation of genomic and miniprep DNA samples |
ssDNA Assay Kit | 1-200 ng | ssDNA quantitation of oligo, primers, denatured DNA, PCR products |
RNA HS Assay Kit | 5-100 ng | RNA quantitation of samples for microarray, RT-PCR, and Northern blot procedures |
RNA BR Assay Kit | 2-1000 ng | RNA quantitation of samples for microarray, RT-PCR, and Northern blot procedures |
microRNA Assay Kit | 0.5–150 ng | microRNA and siRNA quantitation |
Key Features
• Higher sensitivity: 100 times more sensitive than NanoDrop.
• Broad dynamic range: over four orders of magnitude.
• More accuracy: unaffected by the presence of proteins, free nucleotides.
• Easy to use: mix-and-read format.
• Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit®.
Case Study
LiQuant™ DNA Assay Kits exhibit excellent selectivity for DNA, even in the presence of an equal mass of RNA (Left figure); LiQuant™ DNA Quantitation Kits show superior accuracy for detecting dsDNA compared with NanoDrop, especially at relatively low DNA concentrations (Right figure).