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Mycoplasma infections are relatively common in laboratory cell cultures. Mycoplasmas have been shown to alter the growth rate of cells in culture, induce chromosomal aberrations, influence amino acid and nucleic acid metabolism and cause membrane aberrations. Mycoplasma PCR Detection Kit allows for fast and reliable identification of mycoplasma contamination in cell cultures. Mycoplasma DNA in the cell culture supernatant is amplified via PCR and visualized using gel electrophoresis. In addition to the short detection process (less than 2 hours), the easy handling and high sensitivity makes this Mycoplasma PCR Detection Kit a convenient tool for routine examination of cell cultures and media.
The kit contains an optimized PCR master mix and a positive control. The primers in the PCR master mix are highly specific to the conserved rDNA region in the mycoplasma genomes and can detect all well-known mycoplasma genera, including the commonly encountered ones in cell cultures, such as M. orale, M. hyorhinis, M. laidlawii, M. salivarium, M. arginini, M. fermentans, M. hominis, and M. pneumoniae. Mycoplasma positive samples can be easily recognized by a distinctive PCR product ranging in size from 400 to 600 bp.
Key Features
• Simple: Direct addition of cell culture supernatant to PCR reaction, no DNA isolation/purification steps required.
• Fast: Get results in as short as 2 hours.
• Sensitive: Able to detect numerous mycoplasma species.
Applications
Mycoplasma detection
Component
1. 2× PCR Master Mix: 640 µl
2. Positive Control: 45 µl
Storage
Store at -20°C and Avoid repeated freeze-thaw cycles
Case Study
The PCR fragments of the commonly encountered mycoplasmas in cell cultures
