The NK Cells Induction Culture Kit is a culture kit for in vitro culture of NK cells developed in GMP confirming processes, which contains N500 Serum-Free Medium for NK Cells, NK cell activator and NK cell stimulator. This product is a chemically defined, all the compositions of which are produced from medicinal/cell culture grade materials. This product contains no animal component or serum or any other protein component except albumin, transferrin and insulin and can effectively avoid the influence of serum qualitative variation, serum components and exogenous components on experimental research. The optimized formula of this product can significantly improve the proportion, the viability and the killing rate of NK cells in cells cultured in vitro.
Applications
The NK Cells Induction Culture Kit supports the high-density suspension culture of NK cells. In a static culture container, to ensure good gas exchange of the culture system, the recommended depth of the medium is not more than 1.5 cm. The medium supports the high-density culture of NK cells in a bioreactor, and the optimization of the culture procedure is determined by users based on experience. The kit supports the serum free culture of NK cells, and the addition of autologous plasma, human AB serum, FBS or serum substitutes can significantly improve the cell state and increase the cell proliferation rate. Autologous plasma or cell culture additive is recommended to obtain the optimal culture effect.
Components
1. N500 Serum-Free Medium: 1 L
2. NK cell stimulator: 50 μL
3. NK cell activator: 500 μL
Storage
NK cell stimulator and NK cell activator at -25°C~-10°C; N500 Serum-Free Medium at 2~ 8°C and protect from light.