Prestained Protein Marker (2×250 μl)

The protein marker is a multichromatic prestained protein ladder specifically engineered as a dual-functional western blot marker, containing 10 recombinant proteins (10-180 kDa) with three distinct fluorescent signatures (blue/orange/green). This ready-to-load protein marker is formulated in Laemmli buffer with 0.1% SDS, requiring no denaturation or reduction steps prior to SDS-PAGE or western blot applications.
 

Key Features

• Size range: 10 proteins spanning from 10 to 180 kDa.

• Ready-to-use: supplied in a loading buffer for direct loading on gels; no need to boil.

• Sharp bands: color-coded bands of similar intensity for easy visualization.

• Quality tested: each lot evaluated by SDS-PAGE and Western blotting.

• Two reference bands: orange at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.

• Membrane-compatible: colored bands transfer to membranes for Western blotting.
 

Applications

1. Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.

2. Monitoring protein transfer onto membranes after western blotting.

3. Sizing of proteins on SDS-polyacrylamide gels and western blots.
 

Component

1. Prestained Protein Marker: 2×250 μl
 

Storage Buffer

67 mM Tris-H3PO4, pH7.5, 5 mM EDTA, 2% SDS, 33% Glycerol, 0.02% proclin300.
 

Storage

Store at -20°C.

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