RNAprep Pure Cell/Bacteria Kit (50 preps)

The RNAprep Pure Cell/Bacteria Kit provides a fast, simple, and cost-effective method for purification of total RNA from cultured animal cells and bacteria. The purified RNA is ready for use in downstream applications such as: RT-PCR and real-time RT-PCR, gene-chips assay, northern blot, dot blot, polyA screening, in vitro transcript, and molecular cloning.
 

Key Features

• Reliable: Optimized buff ers and protocols for cultured cells and bacteria samples make the process simple and convenient. Unique RNase-Free Filtration Columns CS eliminates other contaminations.

• Efficient removal of gDNA: High-effi cient DNase I is supplied for rapid removal of gDNA on the column.

• Easy and fast: RNA extraction experiments can be completed in 1 hour.

• Safe and low toxicity: No toxic organic reagents such as phenol and chloroform are needed.
 

Applications

1. RT-PCR

2. Northern Blot & Dot Blot

3. Real-Time PCR

4. Chip analysis

5. PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning

Components

1. Buffer RL: 30 ml
2. Buffer RW1: 40 ml
3. Buffer RW: 12 ml
4. RNase-Free ddH2O: 15 ml
5. RNase-Free Columns CR3 set: 50
6. RNase-Free Columns CS set: 50
7. RNase-Free Centrifuge Tubes (1.5 ml): 50

Storage

Store at room temperature.

Case Study

The total RNA of Human Jukat Cells and TOP10 E.coli was isolated using the RNAprep Pure Cell/Bacteria Kit.

Publications

1. Acetylation of xenogeneic silencer H-NS regulates biofilm development through the nitrogen homeostasis regulator in Shewanella. 
Publication: Nucleic Acids Research        Product: RNAprep Pure Cell/Bacteria Kit

2. Regulation of quorum sensing activities by the stringent response gene rsh in sphingomonads is species-specific and culture condition dependent. 
Publication: Frontiers in Microbiology        Product: RNAprep Pure Cell/Bacteria Kit

3. Matrine reverses the resistance of Haemophilus parasuis to cefaclor by inhibiting the mutations in penicillin-binding protein genes ( ftsI and mrcA ). 
Publication: Frontiers in Microbiology        Product: RNAprep Pure Cell/Bacteria Kit