TIANprep Rapid Mini Plasmid Kit (200 preps)

The TIANprep Rapid Mini Plasmid Kit is optimized from traditional alkaline lysis technology, by which high-quality plasmid DNA could be purified within 8 minutes. The new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt. The material which the silica membrane is made of is unique, highly efficient and highly specific. This kit is designed for purification of up to 24 μg plasmid DNA from 1-4 ml overnight cultures of E.coli. The plasmid DNA purified by this kit can be applied in various regular experiments, such as enzyme digestion, PCR, sequencing, ligation, transformation, library screening, in vitro transcription and the transfection of the common sub-culture cells, etc.
 

Key Features

• Fast: Simple procedure with minimal steps to purify high pure plasmid DNA within only 8 min.

• High efficiency: Could purify more than 85% plasmid DNA from E.coli.

• The color indicator TIANRed was supplied to control and remind every process during the experiment to ensure the high efficiency of plasmid purification.

Specifications

Components

1. RNase A (10 mg/ml): 600 μl
2. Buffer P1: 60 ml
3. Buffer P2: 60 ml
4. Buffer P5: 80 ml
5. Buffer PWT: 50 ml
6. Buffer TB: 30 ml
7. TIANRed: 300 μl
8. Spin Columns CP3: 200
9. Collection Tubes 2 ml: 200

Storage

Store at room temperature.

Case Study

Comparison between TIANprep Rapid Mini Plasmid Kit and Supplier A (general plasmidDNA purification kit) by purifying the same plasmid at the same time. Agarose gel electrophoresis shows that TIANprep Rapid Mini Plasmid Kit could obtain same yield of plasmid DNA as that by the general plasmid purification kit within 8 min.

Publications

1. Exo84c-regulated degradation is involved in the normal self-incompatible response in Brassicaceae. 
Publication: Cell reports        Product: TIANprep Rapid Mini Plasmid Kit

2. Regulation of the pleiotropic transcriptional regulator CodY on the conversion of branched-chain amino acids into branched-chain aldehydes in Lactococcus lactis. 
Publication: Applied and Environmental Microbiology        Product: TIANprep Rapid Mini Plasmid Kit

3. Supplementing maternal diet with milk oligosaccharides and probiotics helps develop the immune system and intestinal flora of offsprings. 
Publication: Food Science & Nutrition        Product: TIANprep Rapid Mini Plasmid Kit