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LiFluor™ Violet Cell Proliferation Kit provides convenient single-use vials for cell labeling. The Violet reagent easily diffuses into cells and bind covalently to intracellular amines, resulting in stable, well-retained fluorescent staining that can be fixed with aldehyde fixatives. Excess unconjugated reagent passively diffuses to the extracellular medium, where it can be quenched with complete media and washed away.
The approximate excitation and emission peaks of Violet reagent after hydrolysis are 405 nm and 450 nm, respectively. Cells labeled with Violet reagent can be visualized by fluorescence microscopy using standard DAPI filter sets or analyzed by flow cytometry in an instrument equipped with a 405 nm excitation source.
Key Features
• Superior performance: Bright, single-peak staining enables visualization of multiple generations.
• Long-term signal stability: Well-retained in cells for several days post stain.
• Easy multiplexing with other fluorophores.
• Simple, robust staining protocol.
Specifications
1. Platform: Flow Cytometer
2. Detection Method: Fluorescent
3. Ex/Em: 405/450 nm
Applications
Cell generation analysis by flow cytometry.
Components
1. 10 vials Violet
2. 500 µl anhydrous DMSO
Storage
Store at -20°C and Protect from light.
Cell generation analysis with Violet Cell Proliferation Kit. Jurkat cells (~1×106 cells/ml) were stained with Violet dye (1 µM) on Day 0. The cells were cultured for 7 days. Fluorescence intensity was measured with FACS flow cytometer.
