Exosomes are small vesicles (30–120 nm) containing protein and RNA that are secreted by various types of cells in culture, and found in abundance in body fluids including blood, saliva, urine, and breast milk. Exosomes are thought to function as intercellular messengers, signaling macromolecules between specific cells, however, their formation, and biological pathways in which they are involved remain incompletely understood.
The biological study of exosome function and trafficking requires the isolation of intact exosomes, but the current methods used are tedious, non-specific, and difficult. The Exosome Isolation Reagent from urine provides a simple and reliable method of concentrating intact exosomes from urine samples. By tying up water molecules, the Exosome Isolation Reagent forces less-soluble components (i.e. exosomes) out of solution, allowing them to be collected after brief, low-speed centrifugation.
Key Features
• Maximize recovery of intact exosomes for any type of downstream application.
• Easily isolate exosomes using our simple and reliable protocol.
• Avoid time-consuming ultra-centrifugation.
• Maximize flexibility—can be scaled up or down depending on the sample size.
Component
1. Exosome Isolation Reagent from urine: 50 ml
Storage
Store at room temperature.